Difference between revisions of "Part:BBa K2889003:Design"
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===Source=== | ===Source=== | ||
− | + | We synthesized miR-21-sponge-4s from BBa_K2514000. | |
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+ | https://static.igem.org/mediawiki/parts/e/e6/Amplification_of_miR-21-sponge-4s_fragment.jpeg | ||
===References=== | ===References=== | ||
Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6 | Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6 |
Revision as of 01:21, 9 October 2018
pSB1C3-miR-21-sponge-4s
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 66
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our previous project, we constructed two miR-21 sponge plasmid, pEGFP-miR-21-sponge-2s and pEGFP-miR-21-sponge-6s, as the monitor to detect the expression of miR-21 in cells.The slope of the standard curve of miR-21-sponge-2s is better than miR-21-sponge-6s, suggesting the more sensitive of miR-21-sponge-2s as a monitor. This year, we wanted to improve the sensitive of miRNA sponge. We designed miR-21 sponges contains four miR-21 binding sites with 3-nt spacers for bulged sites based on the sequence of hsa-miR-21 according to the previous study.We constructed pEGFP-miR-21-sponge-4s and pSB1C3-miR-21-sponge-4s this year. Our data suggested miR-21-sponge-4s is more sensitive than miR-21-sponge-2s and miR-21-sponge-6s.
Source
We synthesized miR-21-sponge-4s from BBa_K2514000.
References
Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6