Difference between revisions of "Part:BBa K2810009:Experience"
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− | [[File: | + | [[File:BBa_K2810002_OR_BBa_P10101_AND_K2810009_results.png|700px|thumb|center|Figure 1) The quantification report of the 35S promoter and RTBV promoter when linked to mCherry. This shows the raw photon output of leaf tissue in the table below it too, suggesting that the 35S CaMV promoter has expression 1000x that of the background level, and RTBV between 10-100x background level.]] |
Revision as of 10:18, 5 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2810009
The Cardiff iGEM team of 2018 used this part for quantification promoter sequences in Nicotiana benthamiana, and proved that the part worked in bacteria too. We found that expression in plants was very strong and fairly consistent, making it an excellent reporter gene for use in plants. It was also used to see if the ribitol operon control unit (from E. coli) functioned in plants. Figure 1 shows the results and quantification report when being used to compare the 35S and RTBV promoters. Figure 2 shows the functioning sequence in bacteria (top left) and the use for our collaboration with WashU to characterise their ribitol control operon.
![](/wiki/images/4/41/BBa_K2810002_OR_BBa_P10101_AND_K2810009_results.png)
User Reviews
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Status: 500 Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 252. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 253, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 253, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 263, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 288, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 290. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 316, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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