Difference between revisions of "Part:BBa K2675015"

 
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<partinfo>BBa_K2675015 short</partinfo>
 
<partinfo>BBa_K2675015 short</partinfo>
  
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Synthetic RBS designed for sfGFP
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This part is a synthetic RBS designed for sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) and sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) using the [https://salislab.net/software/forward Salis Lab RBS calculator v2.0] [1, 2].
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used with pAimX-Phi3T short promoter
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This RBS was used to drive the expression of sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) and sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) under the control of the pAimX short promoter ([[Part:BBa_K2675021|BBa_K2675021]]) or its derivative ([[Part:BBa_K2675022|BBa_K2675022]]) in the composite parts [[Part:BBa_K2675051|BBa_K2675051]], [[Part:BBa_K2675061|BBa_K2675061]], ....
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Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
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Translation Initiation Rate (au) : 202865,54
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dG_total (kcal/mol) : -11,34
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dG_mRNA_rRNA (kcal/mol) : -20,08
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dG_spacing (kcal/mol) : 0
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dG_standby (kcal/mol) : 0
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dG_start (kcal/mol) : -2,76
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dG_mRNA (kcal/mol) : -12,28
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Accuracy (warnings) : OK
  
 
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Revision as of 14:06, 7 October 2018


Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the pAimX short promoter (BBa_K2675021) or its derivative (BBa_K2675022) in the composite parts BBa_K2675051, BBa_K2675061, .... 

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	202865,54
dG_total (kcal/mol)	:	-11,34
dG_mRNA_rRNA (kcal/mol)	:	-20,08
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-12,28
Accuracy (warnings)	:	OK

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]