Difference between revisions of "Part:BBa K2540002"
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===Usage and Biology===
 
===Usage and Biology===
 
The broad host range regulatory element was characterized by Johns <i>et al.</i> among other sequences obtained through metagenomic mining. The relative transcription and translation levels measured by Johns <i> et al. </i> are given below:  
 
The broad host range regulatory element was characterized by Johns <i>et al.</i> among other sequences obtained through metagenomic mining. The relative transcription and translation levels measured by Johns <i> et al. </i> are given below:  
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<img src="https://static.igem.org/mediawiki/2018/1/15/T--Rice--HW31422.jpg" width = 50%>
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<p><b>Table 1</b>: Relative transcription and translation levels for the broad host range element (strength 9).</p>
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Revision as of 03:20, 7 October 2018


Broad host range regulatory element (strength 9)

This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 31422) and is originally found in Ilyobacter polytropus.


Usage and Biology

The broad host range regulatory element was characterized by Johns et al. among other sequences obtained through metagenomic mining. The relative transcription and translation levels measured by Johns et al. are given below:



Table 1: Relative transcription and translation levels for the broad host range element (strength 9).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 183


References

Johns, N. I. et al.(2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.