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Revision as of 02:20, 5 October 2018


Broad host range regulatory element (strength 5)

This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 34701) and is originally found in Faecalibacterium prausnitzii.


Usage and Biology

The broad host range regulatory element was characterized by Johns et al. among other regulatory elements obtained through metagenomic mining. The relative transcription and translation rate measured by Johns et al. are given below:



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 183