Difference between revisions of "Part:BBa K2889001:Experience"
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===Applications of BBa_K2889001=== | ===Applications of BBa_K2889001=== | ||
+ | 1.cell migration | ||
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+ | Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS into PCDNA3.1 and transfected the plasmid to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS may play an important role in migration of renal cell carcinoma. | ||
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+ | https://static.igem.org/mediawiki/parts/a/a3/IL7-AS-cell_migration.jpg | ||
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+ | https://static.igem.org/mediawiki/parts/b/b7/IL7-AS-migration-.jpg | ||
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+ | Overexpression of IL7-AS promotes 786-O cells migration. 786-O cells were transfected with pcDNA3.1-IL7-AS for 24 h. The cells growth areas were calculated with Image J at the time points of 0, 6, 12 and 24 h. Bar=100 μm. Data represents means ± SE from 3 independent experiments. *p<0.05 versus non-transfected cells. | ||
===User Reviews=== | ===User Reviews=== |
Revision as of 08:32, 11 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2889001
1.cell migration
Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS into PCDNA3.1 and transfected the plasmid to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS may play an important role in migration of renal cell carcinoma.
Overexpression of IL7-AS promotes 786-O cells migration. 786-O cells were transfected with pcDNA3.1-IL7-AS for 24 h. The cells growth areas were calculated with Image J at the time points of 0, 6, 12 and 24 h. Bar=100 μm. Data represents means ± SE from 3 independent experiments. *p<0.05 versus non-transfected cells.
User Reviews
UNIQcc3db27537981778-partinfo-00000000-QINU UNIQcc3db27537981778-partinfo-00000001-QINU