Difference between revisions of "Part:BBa K2889001:Experience"
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===Applications of BBa_K2889001=== | ===Applications of BBa_K2889001=== | ||
| + | 1.cell migration | ||
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| + | Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS into PCDNA3.1 and transfected the plasmid to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS may play an important role in migration of renal cell carcinoma. | ||
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| + | https://static.igem.org/mediawiki/parts/a/a3/IL7-AS-cell_migration.jpg | ||
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| + | https://static.igem.org/mediawiki/parts/b/b7/IL7-AS-migration-.jpg | ||
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| + | Overexpression of IL7-AS promotes 786-O cells migration. 786-O cells were transfected with pcDNA3.1-IL7-AS for 24 h. The cells growth areas were calculated with Image J at the time points of 0, 6, 12 and 24 h. Bar=100 μm. Data represents means ± SE from 3 independent experiments. *p<0.05 versus non-transfected cells. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 08:32, 11 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2889001
1.cell migration
Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS into PCDNA3.1 and transfected the plasmid to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS may play an important role in migration of renal cell carcinoma.
Overexpression of IL7-AS promotes 786-O cells migration. 786-O cells were transfected with pcDNA3.1-IL7-AS for 24 h. The cells growth areas were calculated with Image J at the time points of 0, 6, 12 and 24 h. Bar=100 μm. Data represents means ± SE from 3 independent experiments. *p<0.05 versus non-transfected cells.
User Reviews
UNIQ12b560d804665f77-partinfo-00000000-QINU UNIQ12b560d804665f77-partinfo-00000001-QINU