Difference between revisions of "Part:BBa K2764001"

Revision as of 06:20, 16 October 2018

Alkane channel protein with BBa_J23104 Constitutive promoter Usage and biology As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764001 utilizes the promoter BBa_J23104, which gives the biobrick a promoter strength of 0.7 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick.

Growth curve <img src="" class="rounded mx-auto d-block" alt="..." width="500px" height="500px">

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 712
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 364
Illegal NgoMIV site found at 577
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2764001 short</partinfo>
+<b>Usage and biology</b>
+As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764001 utilizes the promoter BBa_J23104, which gives the biobrick a promoter strength of 0.7 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick.
--
+<b>Growth curve</b>
+<img src="https://static.igem.org/mediawiki/2018/e/ea/T--Hong_Kong_HKUST--BBa_001growthrate.jpeg" class="rounded mx-auto d-block" alt="..." width="500px" height="500px">
 <!-- Add more about the biology of this part here