Difference between revisions of "Part:BBa K2812006"

Revision as of 07:55, 9 October 2018

Coding sequence for Pyocin S5 with HlyA and His6-tag

Coding sequence for Pyocin S5, which is a bacteriocin produced by a specific strain of P. aeruginosa and is effective against other strains of P. aeruginosa. The sequence encodes for an effector component that consists of a receptor binding domain, a translocation domain and a killing domain. Furthermore it contains an immunity component which gives immunity against the bacteriocin. The working mechanism of Pyocin involves making the target cell membrane permeable, causing leakage of intracellular materials.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 137
Illegal BglII site found at 425
Illegal BglII site found at 2110
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1200

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2812006 short</partinfo>
-Coding sequence for Pyosing S5, which is a bacteriocin produced by a specific strain of P. aeruginosa and is effective against other strains of P. aeruginosa. The sequence encodes for an effector component that consists of a receptor binding domain, a translocation domain and a killing domain. Furthermore it contains an immunity component which gives immunity against the bacteriocin. The working mechanism of Pyosin involves making the target cell membrane permeable, causing leakage of intracellular materials.
+Coding sequence for Pyocin S5, which is a bacteriocin produced by a specific strain of P. aeruginosa and is effective against other strains of P. aeruginosa. The sequence encodes for an effector component that consists of a receptor binding domain, a translocation domain and a killing domain. Furthermore it contains an immunity component which gives immunity against the bacteriocin. The working mechanism of Pyocin involves making the target cell membrane permeable, causing leakage of intracellular materials.
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