Difference between revisions of "Part:BBa K2549010:Design"

(→‎References)
(→‎References)
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Stevens AJ, Brown ZZ, Shah NH, ..., Cowburn D, Muir TW.
 
Stevens AJ, Brown ZZ, Shah NH, ..., Cowburn D, Muir TW.
 
J Am Chem Soc, 2016 Feb;138(7):2162-5  PMID: 26854538; DOI: 10.1021/jacs.5b13528
 
J Am Chem Soc, 2016 Feb;138(7):2162-5  PMID: 26854538; DOI: 10.1021/jacs.5b13528
 +
 
[2]A promiscuous split intein with expanded protein engineering applications.
 
[2]A promiscuous split intein with expanded protein engineering applications.
 
Stevens AJ, Sekar G, Shah NH, ..., Cowburn D, Muir TW.
 
Stevens AJ, Sekar G, Shah NH, ..., Cowburn D, Muir TW.
 
Proc Natl Acad Sci U S A, 2017 Aug;114(32):8538-8543  PMID: 28739907; DOI: 10.1073/pnas.1701083114
 
Proc Natl Acad Sci U S A, 2017 Aug;114(32):8538-8543  PMID: 28739907; DOI: 10.1073/pnas.1701083114

Revision as of 12:54, 3 October 2018


split intein Cfa C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 91
    Illegal SapI.rc site found at 21


Design Notes

The 122-124 residues of Cfa is mutated from EKD to GEP.


Source

IDT(gBlock)

References

[1]Design of a Split Intein with Exceptional Protein Splicing Activity. Stevens AJ, Brown ZZ, Shah NH, ..., Cowburn D, Muir TW. J Am Chem Soc, 2016 Feb;138(7):2162-5 PMID: 26854538; DOI: 10.1021/jacs.5b13528

[2]A promiscuous split intein with expanded protein engineering applications. Stevens AJ, Sekar G, Shah NH, ..., Cowburn D, Muir TW. Proc Natl Acad Sci U S A, 2017 Aug;114(32):8538-8543 PMID: 28739907; DOI: 10.1073/pnas.1701083114