Difference between revisions of "Part:BBa K2623026"

Revision as of 03:55, 14 October 2018

Archaeal ribosomal protein protein L7Ae fused with SpyCatcher.

Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry. It's a non-RFP form of BBa_K2623025 and will be used to construct our total circuit.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1168
Illegal NheI site found at 1191
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2623026 short</partinfo>
-Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we inserted TorA leader sequence to the circuitry.
+Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry. It's a non-RFP form of <partinfo>BBa_K2623025</partinfo> and will be used to construct our total circuit.
 <!-- Add more about the biology of this part here