Difference between revisions of "Part:BBa K2797002"

Line 12: Line 12:
 
[[File:T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg|500px|]]                <div class="service-text">
 
[[File:T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg|500px|]]                <div class="service-text">
 
                     <h3 class="h2">Frank Eardley</h3>
 
                     <h3 class="h2">Frank Eardley</h3>
                     <img src="https://static.igem.org/mediawiki/2018/0/04/T--Newcastle--Frank.png">
+
                     <img src="https://static.igem.org/mediawiki/parts/d/d5/T--NEWCASTLE--SMRCHARACTERISATION.png>
                    <p><br>I am a 4th year MBiol Cellular & Molecular Biology student. I joined iGEM
+
                    for the opportunity to work with students from other academic fields and learn
+
                    more about synthetic biology. Outside of my degree, I am a scuba diving instructor
+
                    and enjoy jumping in the North Sea whatever the weather.</p>
+
 
                      
 
                      
 
                      
 
                      

Revision as of 12:28, 2 October 2018


Composite part for streptomycin resistance

This composite part confers resistance to streptomycin. The part contains the constitutive BBa_J23119 promoter, the strongest promoter of the J23100 through to J23119 promoter family

Part Characterisation

T--NEWCASTLE--STREPTOMYCIN ECOLISMR.jpeg

Figure 1 E. coli strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing streptomycin at a concentration of 50 μg/ml

T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg

Frank Eardley

                   <img src="T--NEWCASTLE--SMRCHARACTERISATION.png>
                   
                   

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 861
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]