Difference between revisions of "Part:BBa K2797002"
Line 12: | Line 12: | ||
[[File:T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg|500px|]] | [[File:T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg|500px|]] | ||
− | <img src="https://static.igem.org/mediawiki/parts/d/d5/T--NEWCASTLE--SMRCHARACTERISATION.png"|500px| | + | <img src="https://static.igem.org/mediawiki/parts/d/d5/T--NEWCASTLE--SMRCHARACTERISATION.png">|500px| |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 12:24, 2 October 2018
Composite part for streptomycin resistance
This composite part confers resistance to streptomycin. The part contains the constitutive BBa_J23119 promoter, the strongest promoter of the J23100 through to J23119 promoter family
Part Characterisation
Figure 1 E. coli strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing streptomycin at a concentration of 50 μg/ml
<img src="">|500px|
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 861 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 709
- 1000COMPATIBLE WITH RFC[1000]