Difference between revisions of "Part:BBa K2669003:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The composite part is equipped with primer binding sites for VF2 and VR and contains Biobrick scar sequence.  
+
The composite part is equipped with primer binding sites for VF2 and VR, prefix and suffix and contains Biobrick scar sequence.  
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
RBS (BBa_J34801), constitutive promotor (BBa_J23119), optimized AmilCP (BBa_K2669002) and double terminator (BBa_B0014) primer binfing sites VF2 and VR (BBa_G00100), (BBa_G00102).  
+
RBS (BBa_J34801), constitutive promotor (BBa_J23119), optimized AmilCP (BBa_K2669002) and double terminator (BBa_B0014), primer binding sites VF2 and VR (BBa_G00100), (BBa_G00102), prefix and suffix in pbs1c3 backbone.  
  
 
===References===
 
===References===

Revision as of 10:39, 2 October 2018


Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part is equipped with primer binding sites for VF2 and VR, prefix and suffix and contains Biobrick scar sequence.


Source

RBS (BBa_J34801), constitutive promotor (BBa_J23119), optimized AmilCP (BBa_K2669002) and double terminator (BBa_B0014), primer binding sites VF2 and VR (BBa_G00100), (BBa_G00102), prefix and suffix in pbs1c3 backbone.

References