Difference between revisions of "Part:BBa K2558001:Design"

Revision as of 05:49, 15 October 2018

lux pR-HS

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

With the hope to find an optimal promotor for our NEON system, we designed 9 mutations on sites 18, 19 and 20 near the luxR binding site and tested one on -10 site that TUST 2017 concluded to show decreased leakage. We conducted experiments to evaluate these lux pR mutants’ reaction to AHL stimulation and their leakage level. The test devices we designed include a constantly expressed luxR and a lux pR (or mutant) (like BBa_K2558211 with original lux pR promotor https://parts.igem.org/Part:BBa_K2558211, and BBa_K2558212 with lux pR-HS promotor https://parts.igem.org/Part:BBa_K2558212). Finally we construct the lux pR-Hypersensitive which contains G19T and TAG44CTT mutations of lux pR promotor.

Source

Mutagenesis of BBa_R0062

References

Koch B et al. The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors. Microbiology. 2005 Nov;151(Pt 11):3589-602.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-G19T and TAG44CTT mutations of lux pR promotor
+With the hope to find an optimal promotor for our NEON system, we designed 9 mutations on sites 18, 19 and 20 near the luxR binding site and tested one on -10 site that TUST 2017 concluded to show decreased leakage. We conducted experiments to evaluate these lux pR mutants’ reaction to AHL stimulation and their leakage level. The test devices we designed include a constantly expressed luxR and a lux pR (or mutant) (like BBa_K2558211 with original lux pR promotor https://parts.igem.org/Part:BBa_K2558211, and BBa_K2558212 with lux pR-HS promotor https://parts.igem.org/Part:BBa_K2558212). Finally we construct the lux pR-Hypersensitive which contains G19T and TAG44CTT mutations of lux pR promotor.
 ===Source===