Difference between revisions of "Part:BBa K2609002"
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<h3>IISc-Bangalore iGEM 2018</h3> | <h3>IISc-Bangalore iGEM 2018</h3> | ||
<p>The free movement of the rest of the phage body against the fixed tail leads to a confirmation change in the baseplate. This confirmation change pulls the entire phage to the cell surface followed by subsequent ejection of the phage DNA into the host<sup>[1]</sup>. The final injection happens with the aid of a "tail tube" that embeds itself into the outer membrane of the host and the mechanism is well conserved across multiple hosts because of similar membrane structures. The first interaction with the cell surface, i.e. the long tail fiber binding, is what determines the specificity of the phage to the host cell<sup>[2]</sup>. Modification of the tip of the tail fiber hence allows for a switching of the receptor that is used by the phage to infect the cell.</p> | <p>The free movement of the rest of the phage body against the fixed tail leads to a confirmation change in the baseplate. This confirmation change pulls the entire phage to the cell surface followed by subsequent ejection of the phage DNA into the host<sup>[1]</sup>. The final injection happens with the aid of a "tail tube" that embeds itself into the outer membrane of the host and the mechanism is well conserved across multiple hosts because of similar membrane structures. The first interaction with the cell surface, i.e. the long tail fiber binding, is what determines the specificity of the phage to the host cell<sup>[2]</sup>. Modification of the tip of the tail fiber hence allows for a switching of the receptor that is used by the phage to infect the cell.</p> | ||
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<img src="https://static.igem.org/mediawiki/parts/8/87/T--IISc-Bangalore--phage_infection_mech.jpg"> | <img src="https://static.igem.org/mediawiki/parts/8/87/T--IISc-Bangalore--phage_infection_mech.jpg"> | ||
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<p>We used an in silico <i><a href="http://2018.igem.org/Team:IISc-Bangalore/PhageModifier">PhageModifier</a></i> pipeline to modify the native gp37 to have increased affinity for phosphoethanolamine. This part is the coding sequence of the first such modification with a predicted binding affinity of ____ kcal/mol.</p> | <p>We used an in silico <i><a href="http://2018.igem.org/Team:IISc-Bangalore/PhageModifier">PhageModifier</a></i> pipeline to modify the native gp37 to have increased affinity for phosphoethanolamine. This part is the coding sequence of the first such modification with a predicted binding affinity of ____ kcal/mol.</p> | ||
Revision as of 19:33, 1 October 2018
T4 bacteriophage long tail fiber protein gp37 (Modification I)
Coding sequence of the long tail fiber protein from T4 bacteriophage. The sequence has been modified in-silico for better binding to phosphoethanolamine near the receptor binding end of the protein. This is the first such modification in our series of four modifications.
Usage and Biology
Biology
The T4 bacteriophage uses its long tail fiber to recognize and bind to its receptor OmpC on the surface of E. coli cells, its cognate host. The binding is mediated by non-covalent interactions that leads to the docking of the tip of the tail fiber (protein gp37) with OmpC in an extremely stable fashion.
This is a modified version of the protein with the following amino acid changes
___
Usage
IISc-Bangalore iGEM 2018
The free movement of the rest of the phage body against the fixed tail leads to a confirmation change in the baseplate. This confirmation change pulls the entire phage to the cell surface followed by subsequent ejection of the phage DNA into the host[1]. The final injection happens with the aid of a "tail tube" that embeds itself into the outer membrane of the host and the mechanism is well conserved across multiple hosts because of similar membrane structures. The first interaction with the cell surface, i.e. the long tail fiber binding, is what determines the specificity of the phage to the host cell[2]. Modification of the tip of the tail fiber hence allows for a switching of the receptor that is used by the phage to infect the cell.
We used an in silico <a href="http://2018.igem.org/Team:IISc-Bangalore/PhageModifier">PhageModifier</a> pipeline to modify the native gp37 to have increased affinity for phosphoethanolamine. This part is the coding sequence of the first such modification with a predicted binding affinity of ____ kcal/mol.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 424
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.
[2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.