Difference between revisions of "Part:BBa K2549003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | When fused with LaG2 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted[1]. | |
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===Source=== | ===Source=== |
Revision as of 10:13, 6 October 2018
anti-GFP (LaG16)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 243
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When fused with LaG2 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted[1].
Source
IDT(gBlock)
References
[1]A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170