Difference between revisions of "Part:BBa J70004"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J70004 short</partinfo> | <partinfo>BBa_J70004 short</partinfo> | ||
− | Cloning plasmid used by | + | Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site. |
Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR. | Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR. |
Revision as of 17:51, 23 January 2008
BioBasic Cloning Plasmid pUC57
Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site.
Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 396
Illegal XbaI site found at 425
Illegal PstI site found at 453 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 396
Illegal PstI site found at 453 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 396
Illegal BamHI site found at 435 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 396
Illegal XbaI site found at 425
Illegal PstI site found at 453 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 396
Illegal XbaI site found at 425
Illegal PstI site found at 453 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1790
Illegal SapI site found at 707