Difference between revisions of "Part:BBa K2549001"

Revision as of 08:42, 6 October 2018

suface-expressed CD19

Surface-expressed CD19(surCD19) is built by fusing CD8α signal peptide and CD19 extracellular domain which has a HA tag on its N terminal and a Myc tag on its C terminal to the transmembrane region of PDGFRβ from N terminal to C terminal. CD8α peptide guides surCD19 to be located to the membrane. Transmembrane region of PDGFRβ embeds it on the plasma membrane. The HA tag and the Myc tag facilitate researchers to detect the expression of the surCD19.It was used as one of our antigens to test the activation of SynNotch.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 588
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 757
Illegal SapI site found at 273
Illegal SapI.rc site found at 91

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 <partinfo>BBa_K2549001 short</partinfo>
-Surface-expressed CD19(surCD19) is built by fusing CD8alpha signal peptide and CD19 extracellular domain which has a HA tag on its N terminal and a Myc tag on its C terminal to the transmembrane region of PDGFRbeta from N terminal to C terminal. CD8alpha peptide guides surCD19 to be located to the membrane. Transmembrane region of PDGFRbeta embeds it on the plasma membrane. The HA tag and the Myc tag facilitate researchers to detect the expression of the surCD19.It was used as one of our antigens to test the activation of SynNotch.
+Surface-expressed CD19(surCD19) is built by fusing CD8α signal peptide and CD19 extracellular domain which has a HA tag on its N terminal and a Myc tag on its C terminal to the transmembrane region of PDGFRβ from N terminal to C terminal. CD8α peptide guides surCD19 to be located to the membrane. Transmembrane region of PDGFRβ embeds it on the plasma membrane. The HA tag and the Myc tag facilitate researchers to detect the expression of the surCD19.It was used as one of our antigens to test the activation of SynNotch.
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