Difference between revisions of "Part:BBa K2705005"
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<partinfo>BBa_K2705005 short</partinfo> | <partinfo>BBa_K2705005 short</partinfo> | ||
| − | This part includes a promoter " | + | This part includes a promoter "PgltAB" and GFP, the expression of GFP is repressed by high level cellular glutamate because ProgltAB is upregulated by GltC, which is repressed by cellular glutamate level. A restriction enzyme KpnI cutting site is between PgltAB and |
| − | + | GFP. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:30, 1 October 2018
PgltAB-GFP
This part includes a promoter "PgltAB" and GFP, the expression of GFP is repressed by high level cellular glutamate because ProgltAB is upregulated by GltC, which is repressed by cellular glutamate level. A restriction enzyme KpnI cutting site is between PgltAB and GFP. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 671
Illegal XhoI site found at 572 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]