Difference between revisions of "Part:BBa K2560259"

Revision as of 15:13, 30 September 2018

mcr gene for Malonyl-CoA Reductase from Sulfolobus tokodaii

mcr encodes the enzyme Malonyl-CoA Reductase from Sulfolobus tokodaii. This enzyme is involved in the 3-hydroxypropionate cycle for CO2 fixation and converts malonyl-CoA into 3-oxopropanoic acid (Strauss & Fuchs, 1993). In a two-step reaction malonyl-CoA is first reduced to 3-oxopropanoic acid and then further reduced to 3-hydroxypropionic acid.

For this part, mcr was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 626
Illegal BglII site found at 819
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2560259 short</partinfo>
-OOOOO
+<i>mcr</i> encodes the enzyme Malonyl-CoA Reductase from <i>Sulfolobus tokodaii</i>. This enzyme is involved in the 3-hydroxypropionate cycle for CO2 fixation and converts malonyl-CoA into 3-oxopropanoic acid (Strauss & Fuchs, 1993). In a two-step reaction malonyl-CoA is first reduced to 3-oxopropanoic acid and then further reduced to 3-hydroxypropionic acid.
+[[File:BBa K2560260 Mcr pathway.png|500px| center]]
+For this part, <i>mcr</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/Part:BBa_K2560002 BBa_K2560002] via BsmBI.
 <!-- Add more about the biology of this part here