Difference between revisions of "Part:BBa K2835000:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
−
This part contains improved version of the <partinfo>BBa_E1010</partinfo> red flourescent protein.  
+
This part is an improved version of <partinfo>BBa_I13502</partinfo>, RBS + mRFP1.  
 
To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
 
To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
  

Revision as of 15:39, 1 October 2018


Enigneered mutant of mRFP1 + RBS (compatible with all iGEM RFC assembly standards)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an improved version of BBa_I13502, RBS + mRFP1. To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.

Part is originally derived from GenBank: AF506027.1

Source

Part is originally derived from GenBank: AF506027.1

https://www.ncbi.nlm.nih.gov/nuccore?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837
from Discosoma striata (coral)

References