Difference between revisions of "Part:BBa K2570002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Site-directed mutation site | |
+ | TyrB(Pst1:CTGCAG—CTGCAA) | ||
+ | sense primer: GGTGGCTGCAATGCTGAATGACG | ||
+ | |||
+ | Anti-sense primer: TCAGCATTGCAGCCACCACCTG | ||
===Source=== | ===Source=== |
Revision as of 09:26, 5 October 2018
TyrB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1054
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Site-directed mutation site
TyrB(Pst1:CTGCAG—CTGCAA)
sense primer: GGTGGCTGCAATGCTGAATGACG
Anti-sense primer: TCAGCATTGCAGCCACCACCTG
Source
Phenylalanine aminotransferase Tyrb from Escherichia coli 21B.