Difference between revisions of "Part:BBa K2807002"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2807002 short</partinfo>
 
<partinfo>BBa_K2807002 short</partinfo>
−
 
+
PspCas13b is a CRISPR-associated protein originating from Prevotella sp and can be expressed in mammalian cells to target and cleave messenger RNA with compatible guide RNA. The sequence also contains a nuclear export signal (NES) and a 3x hemagglutinin (HA) tag.
−
Cas13b is a protein which will include a complementary RNA guide which automatically seeks out specific RNA sequences and promptly destroys the sequences. Cas13b acts as a guide to target pre-selected specific RNA strand.
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:31, 6 October 2018


PspCas13b PspCas13b is a CRISPR-associated protein originating from Prevotella sp and can be expressed in mammalian cells to target and cleave messenger RNA with compatible guide RNA. The sequence also contains a nuclear export signal (NES) and a 3x hemagglutinin (HA) tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1787
    Illegal BamHI site found at 839
    Illegal BamHI site found at 3271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]