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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 05:26, 30 September 2018
KaiC coding region
SUMMARY
It is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites. Therefore, KaiC monomer has four possible phosphorylation states, showing different functions. SasA proteins will affect downstream signaling pathways, when it is phosphorylated by KaiC hexamers in a specific phosphorylated state.
A separate fragment is 1560bp.
The Structure of KaiC
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
Illegal AgeI site found at 859
Illegal AgeI site found at 868
Illegal AgeI site found at 874
Illegal AgeI site found at 1198
Illegal AgeI site found at 1225 - 1000COMPATIBLE WITH RFC[1000]