Difference between revisions of "Part:BBa K2570000:Design"
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The Par (BAG13450.2) the phenyla-cetaldehyde reductase from the rose (Rosa sp.) | The Par (BAG13450.2) the phenyla-cetaldehyde reductase from the rose (Rosa sp.) | ||
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https://www.ncbi.nlm.nih.gov/protein/BAG13450.2?report=genbank&log$=protalign&blast_rank=1&RID=V0NV9P35014 | https://www.ncbi.nlm.nih.gov/protein/BAG13450.2?report=genbank&log$=protalign&blast_rank=1&RID=V0NV9P35014 | ||
Latest revision as of 05:13, 30 September 2018
PAR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 247
Illegal NotI site found at 610 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ordered the part DNA from a synthesis company and had codon optimization of the sequence.
Source
The Par (BAG13450.2) the phenyla-cetaldehyde reductase from the rose (Rosa sp.)
References
1. Chen X M, Kobayashi H, Sakai M, et al. Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol[J]. Journal of Plant Physiology, 2011, 168(2):88-95.
2. Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149.