Difference between revisions of "Part:BBa K2570000"

Revision as of 05:13, 30 September 2018

PAR

To optimize the engineered 2-PE biosynthetic pathway, the Par (BAG13450.2) the phenyla- cetaldehyde reductase from the rose (Rosa sp.)

Construction and optimization of the 2-PE biosynthetic pathway in E.coli.

A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1), phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 247
Illegal NotI site found at 610
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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	Construction and optimization of the 2-PE biosynthetic pathway in E.coli.		Construction and optimization of the 2-PE biosynthetic pathway in E.coli.
		+
	A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1), phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .		A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1), phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .