Difference between revisions of "Part:BBa K2560257:Design"
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The sequence of this part was used from Addgene (Plasmid #55181). To have a small linker to the 3' coding region, a GGG (glycine) was added as well as an ATG (methionine) as startcodon. | The sequence of this part was used from Addgene (Plasmid #55181). To have a small linker to the 3' coding region, a GGG (glycine) was added as well as an ATG (methionine) as startcodon. | ||
| − | The part was codonoptimized for <i>V. natriegens</i> and then synthetisized by IDT and integrated into the vector BBa_K2560002 via BsmBI | + | The part was codonoptimized for <i>V. natriegens</i> and then synthetisized by IDT and integrated into the vector [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560002 BBa_K2560002] via BsmBI |
===References=== | ===References=== | ||
Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765 | Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765 | ||
Revision as of 21:59, 29 September 2018
4x Streptag for N-terminal protein tagging
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To have a small linker to the 3' coding region, a GGG (glycine) was added as well as an ATG (methionine) as startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).
If the Streptag shall be fused to the 5' end (N-terminus of the protein), use this part, for 3' fusion (C-terminus of the protein) use BBa_K2560258.
GGTCTCGAATG-coding_region-GATGTGAGACC
The sequence was codonoptimized for V. natriegens ATCC 14048.
Source
Source of the part:
The sequence of this part was used from Addgene (Plasmid #55181). To have a small linker to the 3' coding region, a GGG (glycine) was added as well as an ATG (methionine) as startcodon.
The part was codonoptimized for V. natriegens and then synthetisized by IDT and integrated into the vector BBa_K2560002 via BsmBI
References
Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765