Difference between revisions of "Part:BBa K2762003:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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The whole genome sequence of <i>Synechococcus elongatus PCC7942</i> is found in NCBI. We first codon optimized the <i>prk</i> sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.
+
The whole genome sequence of <i>Synechococcus elongatus</i> PCC7942 is found in NCBI. We first codon optimized the <i>prk</i> sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.
  
 
===Source===
 
===Source===

Latest revision as of 12:45, 14 October 2018


Phosphoribulokinase (prk)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The whole genome sequence of Synechococcus elongatus PCC7942 is found in NCBI. We first codon optimized the prk sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.

Source

Synechococcus elongatus PCC7942

References