Difference between revisions of "Part:BBa K2856001:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
there is a EcoRI restriction site in gshF
+
there are  EcoRI，XbaI,SpeI restriction sites in original coding squence.So we ask the company to de novo synthsis this squence to remove
−
 
+
the illegal resitriction sites
−
 
+
  
 
===Source===
 
===Source===

Revision as of 07:12, 29 September 2018


Bifunctional glutamate-cysteine ligase/glutathione synthase (gshF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there are EcoRI，XbaI,SpeI restriction sites in original coding squence.So we ask the company to de novo synthsis this squence to remove the illegal resitriction sites

Source

this gene is amplified from Streptococcus thermophilus genome

References