Difference between revisions of "Part:BBa K2762001"

 
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<partinfo>BBa_K2762001 short</partinfo>
 
<partinfo>BBa_K2762001 short</partinfo>
  
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===Usage===
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The rbcX part encode the the chaperon of rbcX RubisCO. The rbcX contribute to the correct folding of the whole RubisCO enzyme.
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===Biology===
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The rbcX genes are from cyanobacteria Synechococcus elongatus PCC 7002. We designed the T7 promoter (BBa_I719005)for the gene and cloned gene in BL21  to ensure the high expression rate. We also did the coden optimization for the gene to ensure successful expression. To get more information, see our composite part: RubisCO
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===Characterization===
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We did two experiment to confirm the expression of this part. First we inserted the part on pSB1C3 and cloned the plasmid into DH5 alpha. We extracted the plasmid after the colony formed and did the enzyme digestion to confirm the insertion was successful. Second, we cloned the plasmid into BL21(DE3) and induced the promoter with IPTG. We than did the SDS PAGE to confirm the present of the  RbcX and RbcS protein.
  
 
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===Usage and Biology===
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Revision as of 06:46, 29 September 2018


Assembly chaperone of ribulose-bisphosphate carboxylase/oxygenase rbcX

Usage

The rbcX part encode the the chaperon of rbcX RubisCO. The rbcX contribute to the correct folding of the whole RubisCO enzyme.

Biology

The rbcX genes are from cyanobacteria Synechococcus elongatus PCC 7002. We designed the T7 promoter (BBa_I719005)for the gene and cloned gene in BL21 to ensure the high expression rate. We also did the coden optimization for the gene to ensure successful expression. To get more information, see our composite part: RubisCO

Characterization

We did two experiment to confirm the expression of this part. First we inserted the part on pSB1C3 and cloned the plasmid into DH5 alpha. We extracted the plasmid after the colony formed and did the enzyme digestion to confirm the insertion was successful. Second, we cloned the plasmid into BL21(DE3) and induced the promoter with IPTG. We than did the SDS PAGE to confirm the present of the RbcX and RbcS protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]