Difference between revisions of "Part:BBa K2560254:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The part contains the whole coding region from the <i>accBC</i> gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). If <i>accBC</i> shall be used with an 5' tag use this part, if there is no need for tagging, use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560251 BBa_K2560251]. <br>
+
The part contains the whole coding region from the <i>accBC</i> gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).  
 +
 
 +
If <i>accBC</i> shall be used with an 5' tag use this part, if there is no need for tagging, use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560251 BBa_K2560251]. <br>
  
 
GGTCTCG<b>GATG</b>-coding_region-<b>GCTT</b>TGAGACC
 
GGTCTCG<b>GATG</b>-coding_region-<b>GCTT</b>TGAGACC

Revision as of 19:21, 28 September 2018


accBC subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 423
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).

If accBC shall be used with an 5' tag use this part, if there is no need for tagging, use BBa_K2560251.

GGTCTCGGATG-coding_region-GCTTTGAGACC


The sequence was codonoptimized for V. natriegens ATCC 14048.

Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0670

Accession number of encoded protein: NP_599932.1

accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.

References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765