Difference between revisions of "Part:BBa K2560253:Design"
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===Design Notes===
 
===Design Notes===
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The part contains the whole coding region from the <i>birA</i> gene of <i>C. glutamicum</i> (NCgl0679) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).
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If <i>birA</i> shall be used with an 5' tag use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560256 BBa_K2560256], if there is no need for tagging, use this part. <br>
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GGTCTCG<b>AATG</b>-coding_region-<b>GCTT</b>TGAGACC
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The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048.
  
  

Revision as of 19:17, 28 September 2018


birA from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the birA gene of C. glutamicum (NCgl0679) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).

If birA shall be used with an 5' tag use BBa_K2560256, if there is no need for tagging, use this part.

GGTCTCGAATG-coding_region-GCTTTGAGACC


The sequence was codonoptimized for V. natriegens ATCC 14048.


Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0679

Accession number of encoded protein: NP_599941.1

birA was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI


References

Samols. D.. Thornton, C.H., Murtif. V.L.. Kumar, G.K.. Haase. F,C. and Wood. H.G. (1988) J. Biol. Chem. 263. 6461-6464.