Difference between revisions of "Part:BBa K2560253"

Revision as of 19:11, 28 September 2018

birA from C. glutamicum, codonoptimized for V. natriegens

birA encodes an biotin-ligase in C. glutamicum. It transfers a biotin to the biotin-carboxylase domain (also known as biotin-carboxyl-carrier protein BCCP) of several biotin-dependent enzymes like Acetyl-CoA Carboxylase and Propionyl-CoA Carboxylase (Samols et al., 1988). Hence this enzyme is involved in fatty acid biosynthesis.

For this part, accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002via BsmBI.

If birA shall be used with an 5' tag use BBa_K2560256, if there is no need for tagging, use this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 132
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2560253 short</partinfo>
-<i>birA</i> encodes an biotin-ligase in <i>C. glutamicum</i>. It transfers a biotin to the biotin-carboxylase domain (also known as biotin-carboxyl-carrier protein BCCP) of several biotin-dependent enzymes like Acetyl-CoA Carboxylase and Propionyl-CoA Carboxylase (Samols et al., 1988). Hence this enzyme is involved in fatty acid biosynthesis. For this part, <i>accBC</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/Part:BBa_K2560002 BBa_K2560002]via BsmBI. If <i>birA</i> shall be used with an 5' tag use [https://parts.igem.org/Part:BBa_K2560256 BBa_K2560256], if there is no need for tagging, use this part.
+<i>birA</i> encodes an biotin-ligase in <i>C. glutamicum</i>. It transfers a biotin to the biotin-carboxylase domain (also known as biotin-carboxyl-carrier protein BCCP) of several biotin-dependent enzymes like Acetyl-CoA Carboxylase and Propionyl-CoA Carboxylase (Samols et al., 1988). Hence this enzyme is involved in fatty acid biosynthesis.
+For this part, <i>accBC</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/Part:BBa_K2560002 BBa_K2560002]via BsmBI.
+If <i>birA</i> shall be used with an 5' tag use [https://parts.igem.org/Part:BBa_K2560256 BBa_K2560256], if there is no need for tagging, use this part.
-Samols. D.. Thornton, C.H., Murtif. V.L.. Kumar, G.K.. Haase.
-F,C. and Wood. H.G. (1988) J. Biol. Chem. 263. 6461-6464.
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