Difference between revisions of "Part:BBa K2623015"

Revision as of 13:19, 29 September 2018

Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator)

TDP circuit is used to express exocrine protein SAHS. In order to facilitate the characterization and purification of SAHS protein, we added the promoter J23100+RBS+SAHS protein+RBS+E1010+B0015 to pSB1C3. TDP circuit is used to express exocrine protein SAHS BBa_K2623007. We added RFP BBa_E1010 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100

and an RBS BBa_B0034 , and followed by a double terminator BBa_B0015.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 279
Illegal XhoI site found at 503
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1152
Illegal AgeI site found at 1264
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 558
Illegal SapI.rc site found at 553

@@ Line 5: / Line 5: @@
 TDP circuit is used to express exocrine protein SAHS.
 In order to facilitate the characterization and purification of SAHS protein, we added the promoter J23100+RBS+SAHS protein+RBS+E1010+B0015 to pSB1C3.
+TDP circuit is used to express exocrine protein SAHS BBa_K2623007.
+We added RFP BBa_E1010 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100
+ and an RBS BBa_B0034 , and followed by a double terminator BBa_B0015.