Difference between revisions of "Part:BBa K2560255:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 9: Line 9:
 
The part contains the whole coding region from the <i>accBC</i> gene of <i>C. glutamicum</i> (NCgl0678) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). If <i>accBC</i> shall be used with an 5' tag use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560254 BBa_K2560254], if there is no need for tagging, use this part.
 
The part contains the whole coding region from the <i>accBC</i> gene of <i>C. glutamicum</i> (NCgl0678) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). If <i>accBC</i> shall be used with an 5' tag use [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560254 BBa_K2560254], if there is no need for tagging, use this part.
  
−
AACGTCTCGCTCG<b>GATG</b>-coding_region-<b>GCTT</b>TGAGGGAGACGAA
+
GGTCTCG<b>GATG</b>-coding_region-<b>GCTT</b>TGAGACC
  
 
The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048.
 
The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048.

Revision as of 18:29, 28 September 2018


accD subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0678) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). If accBC shall be used with an 5' tag use BBa_K2560254, if there is no need for tagging, use this part.

GGTCTCGGATG-coding_region-GCTTTGAGACC

The sequence was codonoptimized for V. natriegens ATCC 14048.

Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0678

Accession number of encoded protein: NP_599940.1

accD was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002via BsmBI

References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765