Difference between revisions of "Part:BBa K2560255:Design"

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===Source===
 
===Source===
  
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Source of the part: Genome: <i>Corynebacterium glutamicum</i> ATCC 13032
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Source of the part:  
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Genome: <i>Corynebacterium glutamicum</i> ATCC 13032
  
 
Accession number of gene: NCgl0678
 
Accession number of gene: NCgl0678

Revision as of 18:24, 28 September 2018


accD subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0678) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011).

If accBC shall be used with an 5' tag use BBa_K2560254, if there is no need for tagging, use this part.

AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA

The sequence was codonoptimized for V. natriegens ATCC 14048.



Source

Source of the part:

Genome: Corynebacterium glutamicum ATCC 13032

Accession number of gene: NCgl0678

Accession number of encoded protein: NP_599940.1

accD was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002via BsmBI

References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765