Difference between revisions of "Part:BBa K2688027:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | In pSB1C3-LEE5*Δtir-gfp, the tir fragment was deleted so that the gfp start codon is at the position of the former tir start codon. Our results indicate that gfp expression is improved when this fragment is removed compared to the parental pSB1C3- LEE5*-gfp plasmid, and that H-NS protein is still able to control the remaining LEE5 region. Of note, Ler proteins are able to activate LEE5 promoter. | + | In pSB1C3-LEE5*Δtir-gfp, the tir fragment was deleted so that the gfp start codon is at the position of the former tir start codon, which yielded BBa_K2688017. The introduced point mutation was then PCR corrected, yielding this biobrick. |
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| + | Our results indicate that gfp expression is improved when this fragment is removed compared to the parental pSB1C3- LEE5*-gfp plasmid, and that H-NS protein is still able to control the remaining LEE5 region. Of note, Ler proteins are able to activate LEE5 promoter. | ||
Revision as of 16:15, 28 September 2018
LEE5_GFP_native_ΔTir
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 50
Illegal BsaI.rc site found at 1003
Design Notes
In pSB1C3-LEE5*Δtir-gfp, the tir fragment was deleted so that the gfp start codon is at the position of the former tir start codon, which yielded BBa_K2688017. The introduced point mutation was then PCR corrected, yielding this biobrick.
Our results indicate that gfp expression is improved when this fragment is removed compared to the parental pSB1C3- LEE5*-gfp plasmid, and that H-NS protein is still able to control the remaining LEE5 region. Of note, Ler proteins are able to activate LEE5 promoter.
Source
LEE5 is a topic of research at the host lab, which provided us with the source plasmid pKK-LEE5-gfp.