Difference between revisions of "Part:BBa K2560251:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The part contains the whole coding region from the accBC gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites. | + | The part contains the whole coding region from the accBC gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). |
AACGTCTCGCTCG<b>AATG</b>-coding_region-<b>GCTT</b>TGAGGGAGACGAA | AACGTCTCGCTCG<b>AATG</b>-coding_region-<b>GCTT</b>TGAGGGAGACGAA | ||
Revision as of 14:25, 28 September 2018
accBC subunit from C. glutamicum, codonoptimized for V. natriegens
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1142
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 423
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0670) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA
The sequence was codonoptimized for V. natriegens ATCC 14048.
Source
Source of the part: Genome: Corynebacterium glutamicum ATCC 13032 Accession number of gene: NCgl0670 Accession number of encoded protein: NP_599932.1 accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsaBI.
References
Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765