Difference between revisions of "Part:BBa K2669001"
m |
m |
||
Line 14: | Line 14: | ||
We have shown that our new improved part does in fact express a histidine tag and has higher expression levels than the old part [[Part:BBa_K2003011]]. | We have shown that our new improved part does in fact express a histidine tag and has higher expression levels than the old part [[Part:BBa_K2003011]]. | ||
− | + | ===Background=== | |
+ | UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments or potentially in environments where bilirubin is naturally present, such as the intestines. | ||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | This part contains the sequence from [[Part:BBa_K2003011]], with a change to the start codon location. The original source of UnaG is from the paper “A Bilirubin-Inducible Fluorescent Protein from Eel Muscle” by Kumagai A et. al, which characterized the UnaG protein from the muscle of a species of Japanese eel. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 10:15, 27 September 2018
Bilirubin-inducible UnaG+6xHis-tag+Flexible linker
WORK IN PROGRESS
This part is a correction of the Uppsala 2016’s Part:BBa_K2003011, which we noticed had a misplaced start codon. This misplaced start codon caused two major problems:
1) This would lead to a reduction in UnaG expression due to a large space being created between the RBS and the start codon
2) This would lead to no histidine tag being expressed
We have shown that our new improved part does in fact express a histidine tag and has higher expression levels than the old part Part:BBa_K2003011.
Background
UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments or potentially in environments where bilirubin is naturally present, such as the intestines.
Source
This part contains the sequence from Part:BBa_K2003011, with a change to the start codon location. The original source of UnaG is from the paper “A Bilirubin-Inducible Fluorescent Protein from Eel Muscle” by Kumagai A et. al, which characterized the UnaG protein from the muscle of a species of Japanese eel.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]