Difference between revisions of "Part:BBa K2560251:Design"
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===Design Notes=== | ===Design Notes=== | ||
The part contains the whole coding region from the accBC gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs for the Marburg Toolbox and a BsmBI recognition site. | The part contains the whole coding region from the accBC gene of <i>C. glutamicum</i> (NCgl0670) without the startcodon. This region is flanked by overhangs for the Marburg Toolbox and a BsmBI recognition site. | ||
| − | + | AACGTCTCGCTCG<b>AATG</b>-coding_region-<b>GCTT</b>TGAGGGAGACGAA | |
The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048. | The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048. | ||
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===Source=== | ===Source=== | ||
Revision as of 14:19, 28 September 2018
accBC subunit from C. glutamicum, codonoptimized for V. natriegens
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1142
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 423
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0670) without the startcodon. This region is flanked by overhangs for the Marburg Toolbox and a BsmBI recognition site. AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAA
The sequence was codonoptimized for V. natriegens ATCC 14048.
Source
Source of the part: Genome: Corynebacterium glutamicum ATCC 13032 Accession number of gene: NCgl0670 Accession number of encoded protein: NP_599932.1 accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsaBI.