Difference between revisions of "Part:BBa K2560007:Design"

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===Design Notes===
 
===Design Notes===
−
The sequence was obtained from part BBa_J23100.
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The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning.
  
 
===Source===
 
===Source===

Revision as of 08:30, 25 September 2018


Phytobrick version of BBa_J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector (BBa_K2560002) using Golden Gate assembly.

Forward Oligo: CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT

Reverse Oligo: CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC

References