Difference between revisions of "Part:BBa K2688023"

 
 
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<partinfo>BBa_K2688023 short</partinfo>
  
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Ler is a nucleoid associated protein encoded by LEE type III secretion system present in some pathogenic E. coli. H-NS and Ler belong to the same family of protein of proteins qualified as ‘silencers’ of foreign DNA expression, the « H-NS family ». However, it has been reported that Ler can counteract the H-NS silencing on the LEE island.
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This protein was used by Leh and collaborators to perform in vitro footprinting assays on LEE5 DNA region, and to analyse the activation of LEE5 promoter in the native enteropathogenic E. coli context by flow cytometry (Leh et al. mBIO, 2017).
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2688023 SequenceAndFeatures</partinfo>

Latest revision as of 16:20, 23 September 2018

tetO_RBS_ler2

Ler is a nucleoid associated protein encoded by LEE type III secretion system present in some pathogenic E. coli. H-NS and Ler belong to the same family of protein of proteins qualified as ‘silencers’ of foreign DNA expression, the « H-NS family ». However, it has been reported that Ler can counteract the H-NS silencing on the LEE island.

This protein was used by Leh and collaborators to perform in vitro footprinting assays on LEE5 DNA region, and to analyse the activation of LEE5 promoter in the native enteropathogenic E. coli context by flow cytometry (Leh et al. mBIO, 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]