Difference between revisions of "Part:BBa K2609005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence was codon optimized for expression in E. coli. The following changes were made to the | + | The sequence was codon optimized for expression in <i>E. coli</i>. |
| − | + | The following changes were made to the wild-type protein sequence. | |
| + | ____ | ||
| + | A double stop codon is placed at the end to ensure proper termination. | ||
===Source=== | ===Source=== | ||
Revision as of 05:42, 2 October 2018
T4 bacteriophage long tail fiber protein gp37 (Modification IV)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 424
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized for expression in E. coli. The following changes were made to the wild-type protein sequence. ____
A double stop codon is placed at the end to ensure proper termination.
Source
T4 bacteriophage genome, sequence from GenBank (https://www.ncbi.nlm.nih.gov/nuccore/HM137666)