Difference between revisions of "Part:BBa K2609003:Design"

 
(Design Notes)
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===Design Notes===
 
===Design Notes===
The sequence was codon optimized for expression in E. coli. The following changes were made to the original sequence. ____
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The sequence was codon optimized for expression in <i>E. coli</i>.
 
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The following changes were made to the wild-type protein sequence.  
 +
____
  
 +
A double stop codon is placed at the end to ensure proper termination.
  
 
===Source===
 
===Source===

Revision as of 05:32, 2 October 2018


T4 bacteriophage long tail fiber protein gp37 (Modification II)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for expression in E. coli. The following changes were made to the wild-type protein sequence. ____

A double stop codon is placed at the end to ensure proper termination.

Source

T4 bacteriophage genome, sequence from GenBank (https://www.ncbi.nlm.nih.gov/nuccore/HM137666)

References