Difference between revisions of "Part:BBa K2587012:Design"
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===Source=== | ===Source=== | ||
− | The sequence for LuxR is obtained from GenBank and codon optimised for E.coli. The remaining parts are from the CIDAR MoClo toolbox. | + | The sequence for LuxR is obtained from GenBank and codon optimised for <i>E.coli</i>. The remaining parts are from the CIDAR MoClo toolbox. |
===References=== | ===References=== |
Revision as of 13:42, 16 October 2018
P_RBS_luxR_T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 260
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 70
Illegal BsaI.rc site found at 701
Design Notes
The construct is optimised for the use with the CIDAR MoClo and contains Type II S restriction sites.
Source
The sequence for LuxR is obtained from GenBank and codon optimised for E.coli. The remaining parts are from the CIDAR MoClo toolbox.