Difference between revisions of "Part:BBa K2632001"

Revision as of 12:51, 27 September 2018

Promoter ent Actived Intracelluraly in Salmonella

Gene ent located in the cluster of enterobactin (ent) genes. In respond to iron deprivation, Salmonella enterica serovar Typhimurim secretes siderophores and enterobactin in SCV condictiont to sense and adapt to the intracellular environment of different types of host cells. Notice that this part carried it's native RBS. You can attach a CDS to it. This part should be in a low copy plasmid such as PSB4K5,or it will express before Salmonella's entry to cell.

Reference

1. Crouch, M. L. V, Castor, M., Karlinsey, J. E., Kalhorn, T. & Fang, F. C. Biosynthesis and IroC-dependent export of the siderophore salmochelin are essential for virulence of Salmonella enterica serovar Typhimurium. Mol. Microbiol. 67, 971–983 (2008). 2.Liu, J., Walsh, C. T., Liu, J., Quinn, N. & Berchtold, G. A. Overexpression, Purification, and Characterization of Isochorismate Synthase (EntC), the First Enzyme Involved in the Biosynthesis of Enterobactin from Chorismate. Biochemistry29, 1417–1425 (1990). 3. Hautefort, I. et al. During infection of epithelial cells Salmonella enterica serovar Typhimurium undergoes a time-dependent transcriptional adaptation that results in simultaneous expression of three type 3 secretion systems. Cell. Microbiol. 10, 958–984 (2008).