Difference between revisions of "Part:BBa K2599008"

Revision as of 10:32, 16 September 2018

T7 Promoter+RBS+Subtilosin+intein+CBD

NCTU_Formosa 2018 designed a composite part encoding the Subtilosin A sequence (BBa_K2599000), and then combined with a T7 promoter (BBa_I712074), a ribosome binding site (BBa_B0034), intein and chintin binding domain (CBD).

Figure 1 biobrick picture

Introduction

Subtilosin A is an antimicrobial peptide produced by Bacillus subtilis. It had bacteriocidal activity against some gram-positive bacteria.

Structure analysis

The amino acid was determined to be as follows:X-Gly-Leu-Gly-Leu-Trp-Gly-Asn-Lys-Gly-Cys-Ala-Thr-Cys-Ser-(sequence; see text) Ile-Gly-Ala-Ala-Cys-Leu-Val-Asp-Gly-Pro-Ile-Pro-Asp-Glx-Ile-Ala-Gly-Ala. The analyses of cross-linking structures revealed that there were linkages between the amino- and carboxyl-termini and between the Cys-19 and the Glx-28 residues through an unknown residue with a residue weight of 163. Consequently, subtilosin A was deduced to be a cyclic peptide antibiotic with a novel cross-linking structure. The production of subtilosin A begins at the end of vegetative growth and finishes before spore formation.

Figure 2 structure picture

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1055
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 778
Illegal AgeI site found at 868
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 698

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2599008 short</partinfo>
-NCTU_Formosa 2018 designed a composite part encoding the Subtilosin sequence [https://parts.igem.org/Part:BBa_K2599000 (BBa_K2599000)], and then combined with a T7 promoter [https://parts.igem.org/Part:BBa_I712074 (BBa_I712074)], a ribosome binding site [https://parts.igem.org/Part:BBa_B0034 (BBa_B0034)], intein and chintin binding domain (CBD).
+NCTU_Formosa 2018 designed a composite part encoding the Subtilosin A sequence [https://parts.igem.org/Part:BBa_K2599000 (BBa_K2599000)], and then combined with a T7 promoter [https://parts.igem.org/Part:BBa_I712074 (BBa_I712074)], a ribosome binding site [https://parts.igem.org/Part:BBa_B0034 (BBa_B0034)], intein and chintin binding domain (CBD).
-<br>
-<br>
-<br>
+Figure 1 biobrick picture
 ===Introduction===
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+Subtilosin A is an antimicrobial peptide produced by <i>Bacillus subtilis</i>. It had bacteriocidal activity against some gram-positive bacteria.
+===Structure analysis===
+The amino acid was determined to be as follows:X-Gly-Leu-Gly-Leu-Trp-Gly-Asn-Lys-Gly-Cys-Ala-Thr-Cys-Ser-(sequence; see text) Ile-Gly-Ala-Ala-Cys-Leu-Val-Asp-Gly-Pro-Ile-Pro-Asp-Glx-Ile-Ala-Gly-Ala. The analyses of cross-linking structures revealed that there were linkages between the amino- and carboxyl-termini and between the Cys-19 and the Glx-28 residues through an unknown residue with a residue weight of 163. Consequently, subtilosin A was deduced to be a cyclic peptide antibiotic with a novel cross-linking structure. The production of subtilosin A begins at the end of vegetative growth and finishes before spore formation.
+Figure 2 structure picture