Difference between revisions of "Part:BBa K2599008"

 
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NCTU_Formosa 2018 designed a composite part encoding the Subtilosin sequence [https://parts.igem.org/Part:BBa_K2599000 (BBa_K2599000)], and then combined with a T7 promoter, a ribosome binding site, intein and chintin binding domain (CBD).
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<h1>'''Introduction'''</h1>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<!-- Add more about the biology of this part here
 
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Revision as of 10:12, 16 September 2018


T7 Promoter+RBS+Subtilosin+intein+CBD

NCTU_Formosa 2018 designed a composite part encoding the Subtilosin sequence (BBa_K2599000), and then combined with a T7 promoter, a ribosome binding site, intein and chintin binding domain (CBD).


Introduction

     


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1055
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 778
    Illegal AgeI site found at 868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 698