Difference between revisions of "Part:BBa K2632002"

 
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Gene sifA is a key gene located in Salmonella Salmonella pathogenicity island and has the function of maintaining the integrity of Salmonella-Containing Vacuole (SCV) and was reported that it expresses after infection.  A fragment including sifA promoter region and its’s RBS region was amplified from Salmonella SL1344 genome. 
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<h3>Reference</h3>
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1.          Garmendia, J., Beuzón, C. R., Ruiz-Albert, J. & Holden, D. W. The roles of SsrA-SsrB and OmpR-EnvZ in the regulation of genes encoding the Salmonella typhimurium SPI-2 type III secretion system. Microbiology 149, 2385–2396 (2003).  3.          Beuzon, C. R. Salmonella maintains the integrity of its intracellular vacuole through the action of SifA. EMBO J. 19, 3235–3249 (2000).                    4.          Malik-Kale, P. et al. Salmonella- at home in the host cell. Front. Microbiol.2, 1–9 (2011)          5.          Steele-Mortimer, O. The Salmonella-containing vacuole-Moving with the times. Curr. Opin. Microbiol. 11, 38–45 (2008)(2008).</p>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2632002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2632002 parameters</partinfo>
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Revision as of 11:28, 16 September 2018

Promoter sifA

Gene sifA is a key gene located in Salmonella Salmonella pathogenicity island and has the function of maintaining the integrity of Salmonella-Containing Vacuole (SCV) and was reported that it expresses after infection. A fragment including sifA promoter region and its’s RBS region was amplified from Salmonella SL1344 genome.

Reference

1. Garmendia, J., Beuzón, C. R., Ruiz-Albert, J. & Holden, D. W. The roles of SsrA-SsrB and OmpR-EnvZ in the regulation of genes encoding the Salmonella typhimurium SPI-2 type III secretion system. Microbiology 149, 2385–2396 (2003). 3. Beuzon, C. R. Salmonella maintains the integrity of its intracellular vacuole through the action of SifA. EMBO J. 19, 3235–3249 (2000). 4. Malik-Kale, P. et al. Salmonella- at home in the host cell. Front. Microbiol.2, 1–9 (2011) 5. Steele-Mortimer, O. The Salmonella-containing vacuole-Moving with the times. Curr. Opin. Microbiol. 11, 38–45 (2008)(2008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]