Difference between revisions of "Part:BBa K2586000:Design"

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===References===
 
===References===
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<a href="https://www.ncbi.nlm.nih.gov/pubmed/28420751">Gundlach<i> et. al. </i> 2017</a>
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[https://www.ncbi.nlm.nih.gov/pubmed/28420751 (Gundlach<i> et. al. </i> 2017)]: Control of potassium homeostasis is an essential function of the second messenger cyclic di-AMP in <i>Bacillus subtilis</i>
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[https://www.ncbi.nlm.nih.gov/pubmed/28420751 (Gundlach<i> et. al. </i> 2017)]
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Gundlach <i> et. al. </i> 2017: Control of potassium homeostasis is an essential function of the second messenger cyclic di-AMP in <i>Bacillus subtilis</i>
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Latest revision as of 07:24, 13 September 2018


Palf4: Constitutively active promoter for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design advantage of the promoter is its length of only 30 bp. This makes it easy to introduce into a genetic system. Another advantage lays in the constitutive expression of the promoter: This allows for many sorts of different experiments.


Source

This part was originally described by Gundlach et. al. (Gundlach et. al. 2017). It was artificially designed to carry out experiments with the soil bacterium and model organism Bacillus subtilis.

References

(Gundlach et. al. 2017): Control of potassium homeostasis is an essential function of the second messenger cyclic di-AMP in Bacillus subtilis